产品货号：
GS2174
中文名称：
XmaI内切酶
英文名称：
XmaI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：XmaI内切酶，XmaI限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Adenovirus -2 DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | C↑CCGGG |
| 来源 | E.coli strain,that carries the cloned gene XmaI from Xanthomonas malvacearum |
| Assayed on | Adenovirus -2 DNA |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 20 mM Tris-HCl(pH 7.6);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 3-fold overdigestion with enzyme 95%of the DNA fragments can be ligated.Of these 90%can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 3 u.a.of enzyme for 16hoursat 37℃ |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | Cfr9I,PspAI,SmaI^,TspMI,XmaCI |
相关搜索：XmaI内切酶，XmaI限制性内切酶